Formalin-Prepared Optic Nerves Case Study - 1422 Words

ll Formalin- prepared optic nerves (ON) was mainly used as the materials and sources of information in the study. To identify the human ON areas where demyelination has occurred, Jennings and Caroll used luxol fast blue (LFB) and hamatoxylin and eosin. Cat ON, on the other hand, were stained with toluidine to distinguish the myelin sheaths in preparation for electron microscopy processing. After this, immunohistochemistry was effectually conducted in order to differentially mark the neuroglia. The digital images formed were then examined and evaluated to quantify the data. Results revealed the presence of oligodendroglial cells in MSON lesions, which further confirms the link between remyelination as a result or consequence of†¦show more content†¦Subpial cortical demyelination (SCD) has been recently found to manifest among 90% of progressive MS patients and is even present to people with early MS. In order to determine whether recurring incidents of inflammatory SCD can si gnificantly change the pattern of oligodendroglial repopulation and lead to demyelinated cortical lesions, the study compared NogoA+ and Olig2+ oligodendrocyte cells. The presence and activities of these cells in patients with early cases and mature stages of MS are examined and carefully analyzed. Results demonstrated a considerable decrease in the NogoA+ and Olig2+ cells for individuals with chronic MS, but those in the early stages of MS showed a different result. Moreover, during the demyelination phase, repeated stimulation of SCD in the experimental rats stemmed to a transitory loss of NogoA+ but did not have a similar effect on the Olig2+ cells. This was followed by the complete repopulation and remyelination of the oligodendroglial cells, notwithstanding the four preceding periods of demyelination. These results indicate successful remyelination in subpial cortical lesions among the rats even after repeated SCD, an indication only apparent to early MS but not to chronic cases. Furthermore, the data obtained demonstrated that the four cycles of continuous demyelination and remyelination process did not effectively sustain an independent rem yelination that has been observed in chronic MS lesions. The results of the